Composite

Part:BBa_K4683012

Designed by: Sahana Narayanan, Kate Sharer   Group: iGEM23_Lambert-GA   (2023-10-11)


hsa-mir-150-5p RCA Padlock Probe

This part is the sequence for the hsa-mir-150-5p Padlock Probe, hsa-mir-150-5p (BBa_K4683009)is used as the basis for the 3' arm (BBa_K4683010) and 5' arm (BBa_K4683011). When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None